解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected by Citrus exocortis viroid (CEVd). Among the differentially expressed proteins detected, 45 were su...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200286
更新日期:2013-03-01 00:00:00
abstract::Using proteomics in tandem with bioinformatics, the secretomes of nonaggressive and aggressive thyroid carcinoma (TC) cell lines were analyzed to detect potential biomarkers for tumor aggressiveness. A panel of nine proteins, activated leukocyte cell adhesion molecule (ALCAM/CD166), tyrosine-protein kinase receptor (A...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200356
更新日期:2013-03-01 00:00:00
abstract::Antibodies that recognize PTMs of histones play a central role in epigenetic proteomic research. Modification-specific antibodies are employed in chromatin immunoprecipitation, for Western blotting and during the immunoprecipitation steps for MS-based global proteomic analyses. Knowledge about the antibodies' off-targ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200383
更新日期:2013-03-01 00:00:00
abstract::The prediction of adverse drug reactions (ADRs) has become increasingly important, due to the rising concern on serious ADRs that can cause drugs to fail to reach or stay in the market. We proposed a framework for predicting ADR profiles by integrating protein-protein interaction (PPI) networks with drug structures. W...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200337
更新日期:2013-01-01 00:00:00
abstract::To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200393
更新日期:2013-01-01 00:00:00
abstract::The ubiquitin-proteasome system allows the targeted degradation of proteins and plays a critical role in the regulation of many cellular processes. Proteasome inhibition is a recent antitumor therapeutic strategy and bortezomib was the first proteasome inhibitor approved for clinical use. In this study, we used the NB...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200233
更新日期:2013-01-01 00:00:00
abstract::Most proteins execute their functions through interacting with other proteins. Thus, understanding protein-protein interactions (PPIs) is essential to decipher biological functions in a living cell. To predict large-scale PPIs, effective and efficient computational approaches are desirable to integrate heterogeneous d...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200326
更新日期:2013-01-01 00:00:00
abstract::Previous studies have found that some first onset schizophrenia patients show signs of impaired insulin signaling. Also, epidemiological studies have shown that periods of suboptimal nutrition including protein deficiencies during pregnancy can lead to increased incidence of metabolic conditions and psychiatric disord...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200376
更新日期:2012-12-01 00:00:00
abstract::Protein arginine methylation is a PTM catalyzed by an evolutionarily conserved family of enzymes called protein arginine methyltransferases (PRMTs), with PRMT1 being the most conserved member of this enzyme family. This modification has emerged to be an important regulator of protein functions. To better understand th...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200132
更新日期:2012-11-01 00:00:00
abstract::Increasing evidence suggests that root extracellular proteins are involved in interactions between roots and their soil environment. In the present study, exudates released by 6-day-old roots of the three legume species white lupin (Lupinus albus), soybean (Glycine max), and cowpea (Vigna sinensis) were collected unde...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100629
更新日期:2012-11-01 00:00:00
abstract::With recent progress in the analysis of the salivary proteome, the number of salivary proteins identified has increased dramatically. However, the physiological functions of many of the newly discovered proteins remain unclear. Closely related to the study of a protein's function is the identification of its interacti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100665
更新日期:2012-11-01 00:00:00
abstract::Plant epidermal trichomes are as varied in morphology as they are in function. In the halophyte Mesembryanthemum crystallinum, specialized trichomes called epidermal bladder cells (EBC) line the surface of leaves and stems, and increase dramatically in size and volume upon plant salt-treatment. These cells have been p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200152
更新日期:2012-09-01 00:00:00
abstract::We report on quality control performed in the context of a large-scale, multi-institutional study of the immune response in blood samples from prostate cancer patients. The measurements were performed by two commercially available techniques/services: protein arrays and an automated bead-based ELISA-like technique on ...
journal_title:Proteomics
pub_type: 杂志文章,多中心研究
doi:10.1002/pmic.201200082
更新日期:2012-09-01 00:00:00
abstract::Separation or prefractionation of proteins by gel electrophoresis, followed by in-gel proteolytic digest and analysis by MS is a typical standard application in proteomics. For many laboratories, it is not cost-effective to use a robot with all its drawbacks. On the other hand, manual digest is time consuming and not ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100354
更新日期:2012-08-01 00:00:00
abstract::Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200070
更新日期:2012-08-01 00:00:00
abstract::Ischemia/reperfusion (I/R) injury is a major consequence of a cardiovascular intervention. The study of changes of the left and right ventricle proteomes from hearts subjected to I/R may be a key to revealing the pathological mechanisms underlying I/R-induced heart contractile dysfunction. Isolated rat hearts were per...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100604
更新日期:2012-08-01 00:00:00
abstract::Previously, we reported that nucleophosmin (NPM) was increased in glioblastoma multiforme (GBM). NPM is a phosphoprotein related to apoptosis, ribosome biogenesis, mitosis, and DNA repair, but details about its function remain unclear. We treated U87MG and A172 cells with small interference RNA (siRNA) and obtained a ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200034
更新日期:2012-08-01 00:00:00
abstract::Plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. Some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergisti...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200048
更新日期:2012-08-01 00:00:00
abstract::Shewanella inhabit a wide variety of niches in nature and can utilize a broad spectrum of electron acceptors under anaerobic conditions. How they modulate their gene expression to adapt is poorly understood. ArcA, homologue of a global regulator controlling hundreds of genes involved in aerobic and anaerobic respirati...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100651
更新日期:2012-06-01 00:00:00
abstract::LTQ Orbitrap data analyzed with ProteinPilot can be further improved by MaxQuant raw data processing, which utilizes precursor-level high mass accuracy data for peak processing and MGF creation. In particular, ProteinPilot results from MaxQuant-processed peaklists for Orbitrap data sets resulted in improved spectral u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100097
更新日期:2012-06-01 00:00:00
abstract::The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better unders...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100496
更新日期:2012-06-01 00:00:00
abstract::Proteolysis affects every protein at some point in its life cycle. Many biomarkers of disease or cancer are stable proteolytic fragments in biological fluids. There is great interest and a challenge in proteolytically modified protein study to identify physiologic protease-substrate relationships and find potential bi...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201200027
更新日期:2012-06-01 00:00:00
abstract::Proteomic analysis on sperm has been restricted to only a few model organisms. We present here a 2DE PAGE proteome map of sperm cells from a nonmodel organism, the marine mussel Mytilus edulis, a free-spawning marine invertebrate with external fertilization. Ninety-six protein spots showing high expression were select...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100500
更新日期:2012-06-01 00:00:00
abstract::Protein interactions have been at the focus of computational biology in recent years. In particular, interest has come from two different communities--structural and systems biology. Here, we will discuss key systems and structural biology methods that have been used for analysis and prediction of protein-protein inte...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100597
更新日期:2012-05-01 00:00:00
abstract::We performed phosphoproteome analysis of proteins from the extremely thermophilic Gram-negative eubacterium Thermus thermophilus HB8 using gel-free mass spectrometric method. We identified 52 phosphopeptides from 48 proteins and determined 46 phosphorylation sites: 30 on serine, 12 on threonine, and 4 on tyrosine. The...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100573
更新日期:2012-05-01 00:00:00
abstract::Proteolytic digestion is an essential step in proteomic sample processing. While this step has traditionally been implemented in homogeneous (solution) format, there is a growing trend to use heterogeneous systems in which the enzyme is immobilized on hydrogels or other solid supports. Here, we introduce the use of im...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100608
更新日期:2012-05-01 00:00:00
abstract::Tectona grandis L. (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree select...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100183
更新日期:2012-04-01 00:00:00
abstract::The presence of high-abundance proteins in complex protein mixtures often masks low-abundance proteins and causes loss of resolution of 2DE. Protein fractionation steps conducted prior to 2DE can enhance the detection of low-abundance proteins and improve the resolution of 2DE. Here, we report a method to prefractiona...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100475
更新日期:2012-04-01 00:00:00
abstract::Selected or multiple reaction monitoring is a targeted mass spectrometry method (S/MRM-MS), in which many peptides are simultaneously and consistently analyzed during a single liquid chromatography-mass spectrometry (LC-S/MRM-MS) measurement. These capabilities make S/MRM-MS an attractive method to monitor a consisten...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100543
更新日期:2012-04-01 00:00:00
abstract::Selected reaction monitoring (SRM) - also known as multiple reaction monitoring (MRM) - has emerged as a promising high-throughput targeted protein quantification technology for candidate biomarker verification and systems biology applications. A major bottleneck for current SRM technology, however, is insufficient se...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100436
更新日期:2012-04-01 00:00:00
abstract::Currently, there are few predictive biomarkers in key biomonitoring species, such as oysters, that can detect heavy metal pollution in coastal waterways. Several attributes make oysters superior to other organisms for positive biomonitoring of heavy metal pollution. In particular, they are filter feeders with a high c...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100417
更新日期:2012-03-01 00:00:00
abstract::We here present the jmzReader library: a collection of Java application programming interfaces (APIs) to parse the most commonly used peak list and XML-based mass spectrometry (MS) data formats: DTA, MS2, MGF, PKL, mzXML, mzData, and mzML (based on the already existing API jmzML). The library is optimized to be used i...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100578
更新日期:2012-03-01 00:00:00
abstract::The community working on model organisms is growing steadily and the number of model organisms for which proteome data are being generated is continuously increasing. To standardize efforts and to make optimal use of proteomics data acquired from model organisms, a new Human Proteome Organisation (HUPO) initiative on ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201290014
更新日期:2012-02-01 00:00:00
abstract::Since its discovery more than 50 years ago, post-translational modification (PTM) of proteins via methylation has grown in prominence, its involvement having been recognised in a number of central processes in the cell. Of these, the best characterised is its role in the epigenetic code. However, there is increasing e...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100397
更新日期:2012-02-01 00:00:00
abstract::The cellular slime mold Dictyostelium discoideum is a soil-living eukaryote, which feeds on microorganisms engulfed by phagocytosis. Axenic laboratory strains have been produced that are able to use liquid growth medium internalized by macropinocytosis as the source of food. To better define the macropinocytosis proce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100313
更新日期:2012-01-01 00:00:00
abstract::Knowledge of gender differences is important because nutritional recommendations on the basis of data collected using predominantly male subjects may not be valid for women. In the present study, we performed proteomic analysis in plasma of rats fed a high-fat diet (HFD) using 2-DE combined with MALDI-TOF-MS for analy...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100127
更新日期:2012-01-01 00:00:00
abstract::The Annual Spring workshop of the HUPO-PSI was this year held at the EMBL International Centre for Advanced Training (EICAT) in Heidelberg, Germany. Delegates briefly reviewed the successes of the group to date. These include the wide spread implementation of the molecular interaction data exchange formats, PSI-MI XML...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.201190117
更新日期:2011-11-01 00:00:00
abstract::Warm ischemia reperfusion (IR) injury of the liver is associated with changes in the expression and/or post-translational modification of numerous proteins. Only a few of these have been identified. We used 2-D DIGE to identify cytosolic proteins altered in the early stage of IR in an established rat model of segmenta...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100053
更新日期:2011-11-01 00:00:00
abstract::The International Protein Index (IPI) database has been one of the most widely used protein databases in MS proteomics approaches. Recently, the closure of IPI in September 2011 was announced. Its recommended replacement is the new UniProt Knowledgebase (UniProtKB) "complete proteome" sets, launched in May 2011. Here,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100363
更新日期:2011-11-01 00:00:00
abstract::Identification and analysis of types of biological protein-protein interactions and their interfaces to predict obligate and non-obligate complexes is a problem that has drawn the attention of the research community in the past few years. In this paper, we propose a prediction approach to predict these two types of co...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100186
更新日期:2011-10-01 00:00:00